Extended Data Fig. 10: Hepatocyte-specific knock-down of Baz2b attenuates MASH pathologies in aged mice.
From: Targeting the chromatin remodeler BAZ2B mitigates hepatic senescence and MASH fibrosis
a-e, Representative images of H&E and ORO staining (a) in liver sections from aged (18-month-old) mice fed with CDAHFD for 4 or 8 weeks and quantitative analysis of liver to body ratio (b), lipid droplets (c), inflammation score (d), ORO+ areas (e), and. Naïve group refers to mice that not injected; shNC group refers to mice that injected with non-targeting control shRNAs; shBaz2b group refers to mice that injected with non-targeting shBaz2b. Scale bars, 50 μm. f, g, Representative staining of SA-β-gal and immunofluorescence staining of p16 and p21 in liver sections from indicated mice (f) and their quantitative analysis (g). h, i, Representative staining of Sirius red and αSMA immunofluorescence (h) in liver sections from aged mice fed with CDAHFD for 8 weeks and their quantitative analysis (i). Scale bars, 50 μm. j, k, Representative staining of CD11b and F4/80 immunofluorescence (j) in liver sections from aged mice fed with CDAHFD for 8 weeks and their quantitative analysis (k). Scale bars, 50 μm. l, Heatmap showing qPCR analysis of changes in the expression of genes related to the Ppara signaling pathway, inflammation, and fibrosis, from the aged mice fed with CDAHFD for 8 weeks. Experiments were repeated three times. Red font highlights DEG with P < 0.05. The numbers of tested mice were indicated in parentheses. Each data point represents a value from one mouse. Data are means ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant; one-way ANOVA with Dunnett’s test was used for determining P values in b-e, g, i, l, k.
