Extended Data Fig. 6: Effects of inhibition of Cd38 on hematopoiesis.
From: Clusterin drives myeloid bias in aged hematopoietic stem cells by regulating mitochondrial function
(a) Scheme for testing the treatment of mice with saline (S) or 78c. (b) Frequency of myeloid, B, and T cells in mice at 4 months after the treatment with saline or 78c (n = 6 per group). (c) FACS plots (left) and quantification (right) of mature B cells (IgM+ IgD+) as the percentage of total B cells in the blood of mice treated with saline or 78c (n = 6 per group). (d) FACS plots (left) and quantification (right) of aged B cells (CD21/CD35−, CD23−) as the percentage of total mature B cells in the blood of mice treated with saline or 78c (n = 6 per group). (e) FACS plots (left) and quantification (right) of naive T cells (CD44− CD62L+) as the percentage of total CD4+ and CD8+ T cells in the blood of mice treated with saline or 78c (n = 6 per group). (f) Physical performance assessed by grip strength, pole test, rotarod test and novel object recognition in mice treated with saline or 78c (n = 8 per group). Unpaired two-tailed Student’s t-test (b, f); One-way ANOVA with Tukey’s multiple comparison test (c–e). Error bars represent mean ± SEM (b–f). Ns: no significant difference.
