Extended Data Fig. 2: Metabolic analysis and breeding performance of control and mutant TrnE mouse models.
a, Summary of birth rates and mutation load for in vivo DdCBE editing at three sites. b–d, Metabolic assessment in 20-week-old and 75-week-old mice. O2 consumption (b) and CO2 elimination (c) are shown for daytime and nighttime. Locomotion (d) is shown as ped meters and total meters (mean ± SEM, n = 9 (20w, control), n = 4–5 (20w, high-mutant TrnE), n = 8 (75w, control and high-mutant TrnE). Unpaired two-tailed Student’s t-test e, Breeding performance (pup number per litter) of TrnE mutant mothers (n = 43) with varying mutation loads, showing no obvious effect on female fertility. The black line (y = mx + c) represents the line of best fit with 95% confidence intervals (gray). f, Total pup number in a 6-month fertility test of control (TrnE 0–17%, n = 4) and high-mutant (TrnE 72–81%, n = 5) TrnE females (mean ± SD, unpaired two-tailed Student’s t-test). g, Male fertility comparison between WT and TrnE high-mutant group. Total pup number over 6 months is shown as the accumulation of litter index. h, Testis weight comparison between control and TrnE high-mutant groups at different ages (3, 8, 24, 50, and 75 weeks) (mean ± SD, n = 3 (3w), n = 5 (8w), n = 7 (24w), n = 8–9 (50w) and n = 6–7 (75w). Unpaired two-tailed Student’s t-test).
