Extended Data Fig. 4: Kidney pathological analysis in mice and evaluation of urinary parameters.
a, Kidney morphology from 100-week-old TrnE male and female mice. The first row shows the mutation load in the tails of the mice, and the second row shows the mutation load in the kidneys. Scale bar = 1 cm. b, PAS staining of the kidneys from 100-week-old male and female mice. The annotated mutation load represents the tail mutation load at the time of dissection. c, Comparison of dilated tubular lumen sizes from b. The size of the dilated tubular lumen was measured as (long axis + short axis) / 2 (mean ± SD, n = 29 (Male), n = 63 (Female). Two-tailed Mann-Whitney test). d, Glomerulus size (μm) quantification from PAS staining data. Kidney tissues were from WT and TrnE high-mutant groups at 50, 75, and 100 weeks (mean ± SD. Two-tailed Mann-Whitney test). e, Quantification of fibrosis-related gene expression via qPCR, ACTB as housekeeping gene (mean ± SD, n = 4. Unpaired two-tailed Student’s t-test). TGFb1, Transforming Growth Factor Beta 1; Vim, Vimentin; Col1, Collagen Type I; Fn, Fibronectin. f, g, Urine protein levels in the control and TrnE high-mutant groups at 50 weeks (f) and 75 weeks (g). Higher protein levels indicate defect in kidney reabsorption function (mean ± SEM, n = 3–5 per group in (f) and n = 7–8 per group in (g). Unpaired two-tailed Student’s t-test). NAG, N-Acetylglucosamine; β2-MG1: β2-Microglobulin 1; ALB, Albumin. h, Urine phosphate levels in the control and TrnE high-mutant groups at 75 weeks (mean ± SD, n = 6. Unpaired two-tailed Student’s t-test). i, Urine glucose levels in the control and TrnE high-mutant groups at 75 weeks (mean ± SD, n = 8. Unpaired two-tailed Student’s t-test). j, Blood glucose levels in the control and TrnE high-mutant groups at 50 and 70 weeks in males and females (mean ± SD, n = 3–5 per group. Unpaired two-tailed Student’s t-test). k, Blood electrolyte levels in the control and TrnE high-mutant groups at 75 weeks (mean ± SD, n = 3. Unpaired two-tailed Student’s t-test).
