Extended Data Fig. 6: TFEB controls resilience in mammalian diapause models.
(a) Representative images of SK-Mel-147 cells treated with siTFEB or siNT control in diapause, or 6 days post INK128 wash out (w/o) either at day 0 of siRNA treatment or 72 hours post siRNA transfection. Scale bar: 100 µm (b) Heat map of wild type killifish tfeb, smad2, smad3b, and smad4b expression from published RNA seq dataset at 9 months of diapause or developing embryos 6 days post fertilization53. z-score. BR = 4. (c) Heat map of wild type killifish tfeb, smad4(1 and 2 of 3) at 3 and 6 days and 1 months of diapause or developing embryos before and without diapause54. z-score. BR = 3. (d) Heat map of wild type killifish tfeb, smad2, smad3b and smad4 expression from adipose tissues of young and old fasted and refed fish. RNA seq samples. z-score. BR = 4. ns = not significant.
