Fig. 5: TFEB controls resilience in mammalian diapause models.
a, sgRNAs counts for mammalian TFEB in the four analyzed conditions: control proliferating mES cells, control proliferating mES cells with doxycycline to induce the Cas9 activity, mES cells in diapause (by treatment with INK128) and mES cells in diapause treated with doxycycline. BR = 3. Two-way ANOVA followed by Tukey’s post hoc test. b, Effect of TFEB silencing on the viability of mES cells, when proliferating and in the diapause-like state. The diapause-like state was induced by treatment with INK128. Each column is normalized on its respective siRNA scrambled. BR = 3. Two-tailed unpaired t-test. c, Effect of TFEB silencing on the viability of SK-Mel-147 cells, when proliferating and in the diapause-like state. The diapause-like state was induced by treatment with INK128. Each column is normalized on its respective siRNA scrambled. BR = 3. Two-tailed unpaired t-test. d, TFEB expression levels from RNA-seq datasets of proliferating compared to diapause-like SK-Mel-147 cells. BR = 4. Two-tailed unpaired t-test. e, Gene-set enrichment analysis enrichment plot of the TGFβ signaling pathway genes in diapause-like SK-Mel-147 compared to proliferating SK-Mel-147. Statistical testing was performed using Kolmogorov–Smirnov-like statistics and adjusted for multiple testing using the Benjamini–Hochberg false discovery rate method. Data and statistics are presented in Supplementary Table 2. NES, normalized expression score.
