Extended Data Fig. 3: HLH-30 regulates TGFβ signaling in response to nutrient cues.
(a) Nuclear/cytosolic hlh-30::mNeonGreen expression in ASI neurons under ARD (2 and 48 hours) and recovery from 48 h ARD (1, 4, 6, and 24 hours of refeeding). Each dot represents one experiment. BR = 3. Mean ± SEM (b) Quantification of daf-7p::GFP fluorescence in OLQ neurons of N2 and hlh-30(tm1078) at 96 hours of ARD. Exposure time 400 ms. Expression in OLQ is 5–10-fold lower than in ASI. Each dot represents the daf-7p::GFP expression in one OLQ neuron. BR = 3, one representative experiment. Mann-Whitney test (two-sided). (c) Schematic of ChIP-qPCR experimental design. (d) Control western blot ChIP-qPCR experiment showing specific elution of HLH-30::FLAG (BR = 2). ‘+’ indicates the use of an Anti-FLAG antibody during IP, ‘-‘ indicates no use of Anti-FLAG antibody (bait). Tubulin control staining was performed on the same gel after washing off the Anti-FLAG antibody. If not stated otherwise: Mean & s.d., One-way ANOVA followed by Turkey’s post-hoc test.
