Extended Data Fig. 4: Regulation of growth signaling protects against senescence.
(a) Distal tip cell (DTC) extension measured from the cap to the end of the most distal process containing lag-2p::mKate2::PH. Each dot represents the mean DTC length of one BR. (b) Representative images of SYGL-1 stained and DAIP labeled germline stem cells (GSCs) at 48 hours of ARD and 24 hours of refeeding. Images show a single z-layer of the germline. Genotypes N2 and hlh-30(tm1978). Arrowhead show the distal end of the gonad. Scale bar 10 μm. (c) Mean SA-β-gal RGB color intensity in the head region of 48 h ARD worms normalized to N2. Genotypes N2, hlh-30(tm1978), hlh-30 daf-1(m40). Each dot represents the SA-β-gal mean intensity of one BR. (d) Mean pharyngeal pumping and (e) Mean body bending rates upon 48 hours of ARD. Genotypes: N2, hlh-30(tm1978), hlh-30 daf-1(m40). 30 second intervals. Each dot represents the mean pumping rate or mean body bends of one BR. (f) Quantification of mitochondrial ROS in N2, hlh-30(tm1078), hlh-30 daf-1(m40) at 48 hours of ARD. Pooled data from three BRs. Kruskal-Wallis followed by Dunn’s post-hoc test. If not stated otherwise: Mean & s.d., One-way ANOVA followed by Turkey’s post-hoc test.
