Extended Data Fig. 5: Rescue of larp-1 mutants by transgenic LARP::GFP and FRAP analysis of LARP-1::GFP.
From: Mitochondria-associated condensates maintain mitochondrial homeostasis and promote lifespan
a-b, Images (a) and quantification (b) of the rescue of defective mitochondria in the hypodermis in larp-1(yq421) mutants by transgenic LARP-1::GFP driven by the col-19 promoter (Pcol-19larp-1::gfp). ≥169 animals were analyzed for each genotype. n = 5 independent experiments. c-d, Images (c) and quantification (d) of the rescue of defective mitochondria in the hypodermis and muscle in larp-1(yq421) mutants by transgenic TOMM-20(1-70)::LARP-1::GFP driven by the larp-1 promoter (Plarp-1tomm-20(1-70)::larp-1::gfp). ≥172 animals were analyzed for each genotype. n = 5 independent experiments. e-f, Images (e) and quantification (f) of the rescue of defective mitochondria in the hypodermis in larp-1(yq421) mutants by transgenic SCAV-3::LARP-1::GFP driven by the larp-1 promoter (Plarp-1scav-3::larp-1::gfp). ≥134 animals were analyzed for each genotype. n = 5 independent experiments. g-h, Left: Time-course images of the recovery of LARP-1::GFP signal in FRAP assays on mitochondria (labeled with TOMM-20::RFP) in the hypodermis (g) and oocyte (h). Photo-bleached regions are framed with dashed lines. Right: Quantification of the recovery of LARP-1::GFP signals. Scale bars, 5 μm. For statistical analyses, P values were determined using two-way ANOVA (b, d, f). Individual data points (mean ± s.e.m.) and exact P values are shown. ***, P < 0.001. NS, not significant (P > 0.05).
