Extended Data Fig. 7: Analysis of LLPS of RBPs with translation machinery proteins.
From: Mitochondria-associated condensates maintain mitochondrial homeostasis and promote lifespan
a, Individual mCh-fused RBPs (3 μM) as indicated were incubated with BFP or BFP-fused translation machinery proteins for 30 min. Images are shown for mCh and BFP fluorescence. Arrows indicate liquid droplets. Bars, 5 μm. b, Overexpression of RBPs failed to rescue the mitochondrial defects in larp-1 mutants. Representative images and quantifications are shown on the left and right, respectively. Bars, 5 μm. ≥181 animals were analyzed for each genotype. n = 6 independent experiments. For statistical analyses, P values were determined using one-way ANOVA (b). Individual data points (mean ± s.e.m.) and exact P values are shown. NS, not significant (P > 0.05).
