Extended Data Fig. 1: Characterization of the larp-1 gene.
From: Mitochondria-associated condensates maintain mitochondrial homeostasis and promote lifespan
a, Schematic representation of the C. elegans larp-1 gene (top), the encoded protein and human LARP1 (bottom). Filled boxes and lines indicate exons and introns in the gene. The yq421 and yq565 mutations are indicated. In C. elegans (C. e) LARP-1 and human (H. s) LARP1 proteins, the LAM (La motif), RRM (RNA recognition motif), and DM15 domains are indicated in different colors. The similarity of each domain between the two proteins is indicated. b, Relative mRNA levels in indicated larp-1 mutants, n = 4 independent experiments for each genotype. c, LARP-1 abundance determined with LC-MS in indicated larp-1 mutants. n = 5 (N2), 4 (larp-1(yq421)) and 4 (larp-1(yq565)) independent experiments. d-e Expression of the larp-1 gene in different tissues. Images (d) of LARP-1::GFP driven by the larp-1 promoter (Plarp-1larp-1::gfp) are shown for the indicated tissues. Boxed regions are magnified (3 ×) in bottom left in merged images. Quantifications of LARP-1::GFP and TOMM-20 intensities along the line in insets of merged images in (d) are shown in (e). f,g, Representative images (f) and quantification (g) of the rescue of defective mitochondria in the hypodermis of larp-1(yq565) mutants by transgenic Plarp-1larp-1::gfp. h,i, Representative images (h) and quantification (i) of the rescue of defective mitochondria in the hypodermis of larp-1(yq421) mutants by human LARP1 (hLARP1) under the control of the larp-1 promoter (Plarp-1hLARP1::gfp). ≥120 animals were analyzed for each genotype. n = 5 independent experiments. Scale bars, 5 μm. For statistical analyses, P values were determined using one-way ANOVA (b-c) and two-way ANOVA (g, i). Individual data points (mean ± s.e.m.) and exact P values are shown. ***, P < 0.001.
