Extended Data Fig. 1: Resident and circulating macrophages are transcriptionally distinct.

(a) Volcano plot depicting the top differentially expressed genes (DEGs) in VAT resident macrophages (F4/80⁺CD11b⁺iv⁻) versus VAT circulating macrophages (F4/80⁺CD11b⁺iv ⁺). Statistical significance was defined at a false discovery rate (FDR) < 0.10 (10%). Genes meeting this threshold are highlighted in red. VAT: Visceral adipose tissue. (b) Volcano plot depicting the top differentially expressed genes (DEGs) in BAT resident macrophages (F4/80⁺ CD11b⁺iv⁻) versus BAT circulating macrophages (F4/80⁺CD11b⁺iv⁺). Statistical significance was defined at a false discovery rate (FDR) < 0.10 (10%). Genes meeting this threshold are highlighted in red. BAT: brown adipose tissue (c) Volcano plot depicting the top differentially expressed genes (DEGs) in VAT resident macrophages (F4/80⁺CD11b⁺iv⁻) versus BAT resident macrophages (F4/80⁺CD11b⁺iv⁻). Statistical significance was defined at a false discovery rate (FDR) < 0.10 (10%). Genes meeting this threshold are highlighted in red. (d) Pathway enrichment analysis of resident macrophages (F4/80⁺CD11b⁺iv⁻) versus circulating macrophages (F4/80⁺CD11b⁺iv⁺) in the VAT. Statistical significance was defined at a false discovery rate (FDR) < 0.10 (10%). (e) Pathway enrichment analysis of resident macrophages (F4/80⁺CD11b⁺iv⁻) versus circulating macrophages (F4/80⁺CD11b⁺iv⁺) in the BAT. Statistical significance was defined at a false discovery rate (FDR) < 0.10 (10%).