Extended Data Fig. 9: Senescence-associated migrasomes propagate senescence signals by activating CD16 on recipient cells.
(a-b) Primary microglia cultures were treated with Aβ40-M or PBS-M for 24 h, with the addition of IgG or anti-CD16 neutralizing antibodies (BD Pharmingen 553142, clone: 2.4G2, 0.5 μg/ml) and/or anti-CD36 neutralizing antibodies (Abcam ab23680, clone: JC63.1, 0.1 μg/ml). The cultures were subjected to western blot analysis to assess the listed senescence markers (a) or Sudan Black staining to reveal the accumulation of lipofuscin (b). Data are presented as mean values +/- SEM. Experiments were repeated three times. Experiments were repeated three times. *P < 0.05, **P < 0.01, ***P < 0.001, compared to PBS IgG group; by one-way ANOVA. (c) Cd5l−/− (AIM KO) RAW264.7 macrophages were generated and stimulated with Aβ40 (20 μg/ml, 24 h). Migrasomes derived from Aβ40-treated AIM KO macrophages were collected and administered to microglia cultures (50 μg/ml, 24 h). WT Aβ40-M were also administered to the cultures along with IgG or anti-CD16 neutralizing antibodies (BD Pharmingen 553142, clone: 2.4G2, 0.5 μg/ml). The cultures were then subjected to western blot analysis to assess the listed senescence markers. Data are presented as mean values +/- SEM. Experiments were repeated three times. *P < 0.05, **P < 0.01, ***P < 0.001, by one-way ANOVA.
