Extended Data Fig. 2: The in situ expression of cellular senescence markers is elevated in 12-month-old neurons, astrocytes, oligodendrocytes and microglia.
(a-d) Coronal brain sections from male mice at 3, 6, 12 and 18 months were subjected to analysis of LMNB1, HMGB1, p-γH2AX, Cenpb, p-STAT3, PLIN2, lipofuscin and SA-β-Gal, followed by co-staining with the marker for neurons (TUJ1, a), astrocytes (GFAP, b), oligodendrocytes (CC1, c) and microglia (IBA1, d). Representative images were displayed. Single channel images were shown in Supplementary Fig. 2. The locations of neurons and astrocytes were demarcated by red dashed lines in images showing intracellular lipofuscin and SA-β-Gal expression. Data are presented as mean values +/- SEM. N = 6 per group. *P < 0.05, **P < 0.01, ***P < 0.001; by one-way ANOVA.
