Extended Data Fig. 3: Relevant correlation analysis of the community cohort, and analysis of Aβ40 accumulation in BAMs of mice.
(a-c) A total of 80 individuals (age = 23–82y) from community population were recruited for the study. (a) Inclusion and exclusion process of the community cohort. (b-c) In the first batch of cohort recruitment, blood samples from 52 individuals (age = 55–82 years) were collected. (b) The association between the percentage of TSPAN4-expressing monocytes or TSPAN4 MFI in monocytes and age, along with the listed parameters in the recruited cohort, was assessed using Spearman correlation analysis. *P < 0.05, **P < 0.01. (c) The association between the percentage of AIM-expressing monocytes or AIM MFI in monocytes and age, along with the listed parameters in the recruited cohort, was assessed using Spearman correlation analysis. *P < 0.05. (d) Coronal brain sections from naïve wild-type (WT) or Tg-SwDI/B+/+ mice of the indicated ages were subjected to Thioflavin-S staining to visualize amyloid tangle accumulation. Experiments were performed four times, and representative confocal microscopic images are shown. (e) The retention of Aβ42, APOE, α-SYN, and MBP in leptomeningeal BAM was assessed using immunostaining. Single channel images were shown in Supplementary Fig. 3c. The percentage of BAMs in the leptomeninges engulfing these substances was calculated, with statistical data presented in Fig. 3a. Experiments were conducted on brain slices from four independent mice, and representative images depicting the accumulation of these substances in leptomeningeal BAMs are displayed. (f) Fluorescent Aβ40-TAMRA (5 μl, 5 mg/ml) was injected into healthy young WT male mice (3 months old) via the cisterna magna (i.c.m.). Mice were sacrificed one day post-injection. Aβ40 engulfment by monocyte-derived macrophages (MoM, F4/80+), microglia (TMEM119+) and BAM (CD206+) was quantified using flow cytometry. Data are presented as mean values +/- SEM. N = 3 in each group. *P < 0.05, **P < 0.01, by one-way ANOVA.
