Extended Data Fig. 2: Complexes of SERPINA3, HP, and ApoE isoforms.
From: Structural signature of plasma proteins classifies the status of Alzheimer’s disease
Complexes were predicted using AlphaFold multimer, based on different sequences of the ApoE isoforms ApoE ε2 (A), ApoE ε3 (C), and ApoE ε4 (E) based on the structure of ApoE ε2, the closest amino acid of HP to the lysine in DVFEEGTEASAATAVKITLL of SERPINA3 was identified and fixed at a distance of 16.3 Å between the two amino acids. For ApoE ε3 and ε4, the distances between the amino acid of HP and the lysine of SERPINA3 were measured to be 15.9 Å and 10.0 Å, respectively. Zoom views are presented for ApoE ε2 (B), ApoE ε3 (D), and ApoE ε4 (F). Predicted SERINA3 is displayed in red, HP in cyan, and ApoE in purple. The green represents the sequence (DVFEEGTEASAATAVKITLL) of SERPINA3. The experimentally determined structure of SERPINA3 is in grey. Contact density (G), interface area (H), and binding affinity (I) between the proteins were calculated.
