Fig. 4: Maximum-likelihood phylogeny of methyl-coenzyme M reductase submit alpha (McrA) of archaea.

This tree was inferred using IQ-TREE v1.6.10 with LG + F + I + G4 as the best-fit evolutionary model and 1000 ultrafast bootstraps based on an alignment of 450 amino acid positions of 167 sequences from diverse MCR-containing archaea. The McrA sequences of Helarchaeota were placed into three distinct clusters: Hel Cluster I, II, and III. The scale bar shows estimated sequence substitutions per residue.