Fig. 4: Comparative analysis between in vitro and in vivo [11] including both adults and elderly. | ISME Communications

Fig. 4: Comparative analysis between in vitro and in vivo [11] including both adults and elderly.

From: In vitro metabolic capacity of carbohydrate degradation by intestinal microbiota of adults and pre-frail elderly

Fig. 4

A First axis of the principal response curve showing alterations in microbial composition over time in response to GOS in vivo and in response to non-carbohydrate control (protein mix) and carbohydrates (GOS or 2′-FL) in vitro, while taking in vivo GOS intervention as reference. Both duplicate samples were included for the analysis. Genera for which the model best explained the observed variation between reference and treatments (weights > 0.05) are shown on the right side of the figure. B Relative abundance of different bacterial families (top 12, ranked based on the average relative abundance across the entire dataset) in the microbiota of six adults and six elderly. Averaged relative abundance of the duplicate samples was used here (B) for visibility. Top 12 microbial families are listed in the legend. Other families are summarized as “Other”. Each column represents the corresponding type of sample from one subject. Samples collected at 24 h were excluded from this comparative analysis as some carbohydrates were completely depleted within 10 h (see Figs. 2 and 3). AD adult, EL elderly, GOS galacto-oligosaccharides, 2′-FL 2′-fucosyllactose.

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