Fig. 3: Differences in relative transcription levels of genes involved in organic N degradation.
From: Increased microbial expression of organic nitrogen cycling genes in long-term warmed grassland soils
The relative transcription levels of all genes encoding secreted proteins putatively involved in organic N degradation in relation to all protein-coding genes in each sample are shown as transcripts per million (TPM) between ambient and warmed soil plots of the medium-term warmed grassland (MTW) and the long-term warmed grassland (LTW) (A). Inside the boxplots, the median value is shown. A two-way ANOVA was used to assess statistical the statistical significance. Subplot (B) shows a nonmetric multidimensional scaling (NMDS) ordination of all samples, according to the transcribed functions under analysis. The percentage of variation explained by each factor in a PERMANOVA is shown in the NMDS plot. Unpaired t-tests (two-tailed) were used after checking the parametric assumptions, and p-values were adjusted for the false-discovery rate (Benjamin-Hochberg correction). Detailed statistical results can be found in Table S7.
