Fig. 2

Detection efficiencies of three probe sets targeting GH92 genes with direct-geneFISH in pure cultures of Polaribacter Hel1_33_49 and Formosa Hel1_33_131. A POL-GH92_a in Polaribacter was targeted with Alexa 488-labeled polynucleotide probes (yellow) and the 16S rRNA with the species-specific probe POL183a (cyan). B FORM-GH92_a in Formosa was targeted with Alexa 594-labeled polynucleotide probes (yellow), FORM-GH92_b with Alexa 647-labeled polynucleotide probes (magenta) and the 16S rRNA with a species-specific probe FORM181B or general bacterial probe EUB338I-III (cyan). The DNA was counterstained with DAPI (appears blue in “all channels”). Shown micrographs are maximum intensity projections of processed images achieved with Airyscan microscopy in super-resolution mode. NC = negative control, ns = not significant, scale bar: 1 µm, error bars in histograms represent standard deviations of the mean efficiencies. Experimental details are given in Table 1 and Supplementary Table 4.