Fig. 2: Weekly dynamics and seasonal patterns for major phytoplankton groups observed over the 4-yr time series in Bedford Basin (Halifax, NS).

Sequences are chloroplast 16S plus cyanobacterial 16S. a weekly relative abundances for all ASVs at 5 m based on their assignment to a major taxonomic group; values shown relative to nutrients, chl a and temperature [see Fig. S5 for 1 & 10 m depths]. Unrarefied data (shown in figure) had identical trends to rarefied data, yet unrarefied data retained more samples [compare Figs. S5, S6]. White columns represent missing or unsuccessfully sequenced samples, or those that only had bacterial 16S reads; for our 1–60 m Bedford Basin datasets 9 (V4-V5) and 23 (V6-V8) samples only yielded bacterial 16S data, and 2 (V4-V5) and 5 (V6-V8) samples did not yield any final sequence reads post processing (Supplementary Data S5–S7). b Summary bar-plots comparing total relative abundance of broader groupings per season and year [going from Feb-01 to May-01 and Aug-01 to Nov-01], data shown are rarified to 200 reads and include all ASVs at 1–10 m depths. c Distributions of Bray-Curtis similarities between 5 m samples plotted against the number of weeks separating samples [relative abundances used from rarefied data; values were also Hellinger transformed [47]].