Fig. 2: Prevalence-based model for deriving acidification-related gain/loss of metagenomic functions. | ISME Communications

Fig. 2: Prevalence-based model for deriving acidification-related gain/loss of metagenomic functions.

From: Metagenomic shifts in mucus, tissue and skeleton of the coral Balanophyllia europaea living along a natural CO2 gradient

Fig. 2

KEGG orthologs (KO genes) List of KO entries (KEGG orthologs) in the metagenome obtained from the 3 B. europaea anatomic compartments (mucus, soft tissue, and skeleton) in different acidification conditions (S1, control site; S2, moderate acidification; S3, high acidification). KO genes showing a prevalence of 100% (i.e., detected in 3 out of 3 replicates) in S1 and 0% (i.e., none of the 3 replicates) in S3 or vice versa, are reported, identifying functions lost or gained with acidification, respectively. A heatplot with a color gradient corresponding to the number of replicates in which each KO is detected is provided (see legend top-right for color key). For each KO, listed with KO identification number (left), the KEGG description is given on the right of the heatplot. KO entries are grouped by coherence of involvement in different physiological functions (i.e., genes classified as involved in transcriptional regulation, membrane transport, membrane or cell wall functionality maintenance, stress response, nitrogen (N) metabolism, chorismate pathway, methane (CH4) metabolism), as well as by their increasing or decreasing trends among acidification conditions in the same coral compartment, whenever possible.

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