Fig. 1: Diagram illustrating RoC-ITS strategy.
From: High-resolution phylogenetic and population genetic analysis of microbial communities with RoC-ITS
The 16S-ITS region is isolated using PCR. Gibson Assembly is then used to circularize the PCR products. A long single-stranded DNA product encompassing multiple iterations around the circular template is then generated using the phi29 polymerase. The linear product is finally modified by constructing a double-stranded A-tailed end (Nanopore Library Adapter). At this point, the product is ready for normal library construction using a Nanopore DNA library kit.
