Fig. 4: Phylogenetic analysis of the Bacillus subtilis RoC-ITS sequences. | ISME Communications

Fig. 4: Phylogenetic analysis of the Bacillus subtilis RoC-ITS sequences.

From: High-resolution phylogenetic and population genetic analysis of microbial communities with RoC-ITS

Fig. 4

The tree scale shows the number of edits per base per unit length. a Phylogenetic tree of Bacillus RoC-ITS sequences along with the 10 reference Bacillus subtilus str 168 rrns (labeled CP052842.A-J with the letters corresponding to their order in ascending base pair coordinates on the reference genome). The RoC-ITS reads are found in eight distinct clades in accordance with the reference rrns. The single largest clade containing three reference rrns (F, G, and I) and consists of the two identical rrns plus a third rrn that differs by only two deleted bases. b A phylogenetic tree of the reference rrns and the consensus sequences derived from the nine clusters produced after clustering the Bacillus RoC-ITS sequences at 99.55% identity.

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