Fig. 3: Clustering and distribution of trait attributes across EBPR SBR community members.

Using the TbasCO method, we identified expression-based trait attributes from predefined trait modules in the KEGG library and explored the distribution of these trait attributes across community members. A Distribution of trait-attributes among sets of genomes. Bars represent the number of trait-attributes present in a set number of genomes and colored by KEGG module category. Among a total of 35 genomes, trait attributes present between 3 and 18 genomes are designated as niche differentiating, whereas trait attributes present in 19 or greater genomes are designated as core trait attributes. Inset figure demonstrates the maximum number of attributes for the maximum number of genomes. B Cytoscape network showing the connectedness of genomes to trait attributes. The network was filtered to only include nodes with more than 5 connections, therefore filtering out both genomes with few trait attributes and trait attributes connected to less than 5 genomes. Genomes are represented as squares colored by phylum, and trait attributes are represented as circles colored by KEGG category. The size of both the squares and circles represents the number of connections to that genome or trait attribute, respectively.