Fig. 5: Expression dynamics of distributed denitrification routes.

Expression of denitrification traits distributed among community members in the EBPR SBR ecosystem. Timepoints 1–3 correspond to the anaerobic phase and timepoints 4–6 correspond to the aerobic phase as referenced in Fig. 1. A Complete denitrification pathway and associated genetic repertoire with each sequential step. B Trait attributes of expression dynamics for community members with the narGH nitrate reductase system. This trait was the only denitrification trait identified with more than one attribute. C Expression dynamics of the napAB nitrate reductase system. D Expression dynamics of the norBC nitrous oxide reductase system. E Expression of all steps of denitrification starting at nitrite reduction. F Expression of the most complete denitrification route among three community members, with the norC subunit for nitrous oxide reduction missing. Note that OTTO1 only contains nirS but is included in this trait attribute because the expression dynamics are similar to that of the other three genomes for this subunit.