Fig. 1: Quantification of pig and bacterial DNA in NS-MAG, MOLZ and MOLZ-F extracts obtained from samples following four storage conditions. | ISME Communications

Fig. 1: Quantification of pig and bacterial DNA in NS-MAG, MOLZ and MOLZ-F extracts obtained from samples following four storage conditions.

From: Effect of bacterial DNA enrichment on detection and quantification of bacteria in an infected tissue model by metagenomic next-generation sequencing

Fig. 1

A Pig DNA quantified by qPCR. B Bacterial 16S rRNA gene copy number quantified by qPCR. C Bacterial 16S rRNA gene copy number (as in B) normalized per pig DNA mass (as in A). D Ratio of bacterial-to-pig sequencing reads in the mNGS dataset. The number of read pairs corresponding to pig genome was identified by CLARK. For bacteria, we calculated the average for the number of forward and reverse reads mapped by USEARCH to genome sequence assemblies of the four mock-community species.

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