Fig. 2: Horizontal communities are enriched in xenotypic sequences.

a Unique contigs within horizontal (blue) and vertical (orange) communities identified by xenoseq_find. The solid lines represent the mean across 10 communities, shaded areas represents the standard error. b Aligning contigs identified by xenoseq_find to ancestral samples allows linking of donor-acceptor pairs (example network shown for horizontal and vertical communities at week 40, also see Supplementary Figs. 1+2). For horizontal communities, links in the network represent candidate elements that are horizontally transferred. For vertical communities, the links instead represent false positives, as transfer from allopatric communities is excluded by the experimental design. c The number of xenotypic contigs (sequences linked to at least one donor community) identified in horizontal communities is shown in blue, and false positives observed in vertical communities is shown in orange. Dots represent 10 different replicate communities sampled at each time point, the solid lines represent the mean a cross communities, and the shaded area represents the standard error.