Fig. 6: Microbial populations show distinct patterns in distribution and activity across samples.

The tree was calculated from a concatenated alignment of 16 single-copy ribosomal protein genes (SC-RBPs), with a threshold of at least 8 genes per MAG for inclusion in the tree. Relative abundance of MAGs was defined as the quotient between the truncated average sequencing depth of the MAG and the average sequencing depth of 16 SC-RBPs in the sample. Relative proportion of transcription was defined by the average TPM value of the MAG-encoded 16 SC-RBPs and the whole sample average TPM values for the 16 SC-RBPs.