Fig. 6: The Wood–Ljungdahl pathway summary of carbon fixation MAGs.
From: Phylogenetically and metabolically diverse autotrophs in the world’s deepest blue hole
The WL pathway consists of the carbonyl and methyl branches that reversibly reduce CO2 to acetyl-CoA. THF and H4MPT generally regarded as two versions of the methyl branch. The complete WL pathway was considered present only if all of the genes encoding at least one full enzymatic pathway capable of carrying out that metabolic process were found in genomes. These MAGs all contained the genes of carbonyl-branch. Blue and pink represent H4MPT and THF methyl-branch, respectively. The bold pink Bins (Bin23, Bin144) represent that harbored complete H4MPT methyl-branch, other regular pink Bins represent that lacked the fdh gene at first step but have alternative formate dehydrogenase subunits. Thus, a total of ten Bins could contain the complete WL pathway. The bold yellow Bins represent that can generate acetyl-CoA to acetic acid via the complete WL pathway and phosphate acetyltransferase-acetate kinase pathway.
