Fig. 3: miR-17-20a induces ischemic angiogenesis and an M2-like macrophage phenotype. | Communications Medicine

Fig. 3: miR-17-20a induces ischemic angiogenesis and an M2-like macrophage phenotype.

From: Inhibiting anti-angiogenic VEGF165b activates a miR-17-20a-Calcipressin-3 pathway that revascularizes ischemic muscle in peripheral artery disease

Fig. 3

a In vitro tube formation assay of normal HUVECs transfected with negative inhibitor (Neg-Inh, blue bars, n = 4), miR-17 inhibitor (miR-17 Inh, yellow bars, n = 5) or miR-20a inhibitor (miR-20a Inh, pink bars, n = 6) on growth factor-reduced Matrigel (GFRM). Scale bars are 50 µm. One-way ANOVA with Dunnett’s post-test. b In vitro tube formation assay of HSS-HUVECs transfected with Neg-Mim (blue bars, n = 4), miR-17-Mim (yellow bars, n = 6), or miR-20a-Mim (pink bars, n = 6) on GFRM. n = 6. Scale bars are 50 µm. One-way ANOVA with Dunnett’s post-test. c qPCR of arginase-1 (Arg1) and inducible nitric oxide synthase (iNOS) expression in normal BMDMs transfected with negative inhibitor (Neg-Inh, blue bars), miR-17 inhibitor (miR-17 Inh, yellow bars,) or miR-20a inhibitor (miR-20a Inh, pink bars). n = 6. Unpaired t-test. d qPCR analysis of Arg1 and iNOS expression in HSS-BMDMs transfected with Neg-Mim (blue bars), miR-17-Mim (yellow bars), or miR-20a-Mim (pink bars). n = 6. Unpaired t-test. Outliers were removed by performing the Grubbs test. P < 0.05 significant. Data from the biological replicates are presented as mean ± standard error.

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