Fig. 6: RCAN3 inhibits ischemic angiogenesis and induces an M1-like macrophage phenotype in vitro. | Communications Medicine

Fig. 6: RCAN3 inhibits ischemic angiogenesis and induces an M1-like macrophage phenotype in vitro.

From: Inhibiting anti-angiogenic VEGF165b activates a miR-17-20a-Calcipressin-3 pathway that revascularizes ischemic muscle in peripheral artery disease

Fig. 6

qPCR analysis and western blot analysis of RCAN3 in a BMDMs (qPCR: normal n = 4 (blue bar), HSS n = 4 (pink bar); Western blot: normal n = 3 (blue bar), HSS n = 3 (pink bar), Unpaired t-test, b SkMVECs (qPCR: normal n = 6 (blue bar), HSS n = 6/time point (pink bars); Western blot: normal n = 3 (blue bar), HSS n = 3 (pink bar)), one-way ANOVA with Dunnett’s post-test for qPCR analysis, Unpaired t-test for western blot analysis, and c HUVECs (qPCR: normal n = 6 (blue bar), HSS n = 6/time point (pink bars), Western blot: normal n = 3 (blue bar), HSS n = 3(pink bar)), one-way ANOVA with Dunnett’s post-test for qPCR analysis, Unpaired-t-test for western blot analysis. d In vitro tube formation assay of HSS-HUVECs transfected with Neg Pld (blue bar) or RCAN3 plasmid (pink bar) on GFRM. n = 4. Scale bars are 50 µm. Unpaired t-test. e qPCR of arginase-1 (Arg1, (Unpaired t-test with Welch’s correction)) and inducible nitric oxide synthase (iNOS) expression in HSS-BMDMs transfected with Neg Pld (blue bar) or RCAN3 Pld (pink bar), n = 6. Unpaired t-test. P < 0.05 significant. Data from the biological replicates are presented as mean ± standard error.

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