Fig. 6: Effect of TH analogs on the expression of THR-regulated genes and lipid metabolism-related genes in the liver.
Total RNA was extracted from frozen liver tissue from mice fed with a high-fat diet administered either saline, T3, GC-1, or ZTA-261 at 1 µmol/kg day, and subjected to RT-qPCR analysis. Relative expression levels of Dio1 (a), Me1 (b), Thrsp (c), Cyp7a1 (d), Ldlr (e), Srebp1c (f), and Pnpla2 (g) were determined by Pfaffl’s method using Rsp18 as a reference gene. Data are shown as box plots, with median, 25th and 75th percentiles and maximum and minimum values (n = 9–10). Significant differences among the groups are indicated by different letters Significant differences among groups are indicated by asterisks (*P < 0.05, **P < 0.01, and ***P < 0.001, Kruskal–Wallis test with Dunn’s multiple comparisons test).
