Fig. 3: Host factor PLAC8 is required for efficient infection by Spike-typed pseudoviruses.
From: Host factor PLAC8 is required for pancreas infection by SARS-CoV-2
a Susceptibility to infection with Spike-typed (corresponding to Wuhan-1, BA.1 or BA4/5 variants-of-concern) or empty vector-typed lentiviruses in exocrine pancreas cell lines MiaPaca-2, HPAF-II and SUIT-2 CRISPR knock-out (KO) cell lines with loss-of-function of PLAC8. NT is a non-targeting CRISPR control. KO was confirmed by Western Blot. Bars represent the average and standard error of the mean (SEM) of the ratio of infected cells in each condition normalized to the non-targeting control cell line NT; b Rescue experiments in PLAC8 in SUIT-2 KO cell lines: bar plot showing the average and SEM of the ratio of infection (normalized to NT with GFP-empty vector overexpression) of Wuhan-1 Spike-typed lentivirus in PLAC8-KO1 and KO2 that overexpress CRISPR-resistant GFP-PLAC8 construct. Knock-out and rescue were confirmed by Western Blot. The number of independent biological replicates (n) is indicated. The significance above each bar represents the t-test p-value between indicated conditions. ns, P-value ≥ 0.05, *P-value < 0.05, **P-value < 0.01, ***P-value < 0.001.
