Fig. 3: Infrared microscopy results.
Violin plots showing the distribution of the relative intensities of the following bands: a Amide I (AI; 1714–1585 cm-1); b Amide II (AII; 1585–1483 cm-1); c Phosphate I (PhI; 1270–1186 cm-1); d Phosphate II (PhII; 1135–1004 cm-1); e C=O carbonyl ester band (CO; 1760–1718 cm-1) with respect to the integration over the 1800–950 cm-1 spectral range, and f of the asymmetric CH2 with respect to the asymmetric CH3 spectral ratio (aCH2/aCH3; 2945–2900 cm−1 and 2980–2945 cm−1, respectively). As indicated in the figure, the total number of animals considered is 3 (N = 3). Each plot contains more than 3000 points corresponding to the infrared spectrum in each pixel of the brain sections of the animals. Due to the large number of points per animal, they have been omitted from the graph for a correct visualization. The nonparametric Kruskal–Wallis test was employed to assess the significance between the control and irradiated groups. Then, when global group effects were found to be statistically significant, a Dunn test for pairwise comparisons, including the Bonferroni adjustment, was performed. Gy refers to Gray.
