Fig. 1: Addition of kifunensine during production in CHO cells affects spike trimer glycosylation. | Communications Medicine

Fig. 1: Addition of kifunensine during production in CHO cells affects spike trimer glycosylation.

From: Modifying the glycosylation profile of SARS-CoV-2 spike-based subunit vaccines alters focusing of the humoral immune response in a mouse model

Fig. 1

a Schematic of construct design for recombinant SARS-CoV-2 spike proteins used in the current study. b Reduced ( + DTT) and non-reduced (-DTT) SDS-PAGE analysis, vertically separated numbers represent ladder separation in kDa, c monosaccharide analysis (HPAEC-PAD; mean of n = 3 technical replicates shown by open circles), and d analytical size exclusion chromatography (representative of multiple runs).

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