Fig. 3: SLA archaeosome-adjuvanted T4 foldon-trimerized SARS-CoV-2 spike with complex type (CT) N-linked glycans induces enhanced neutralizing responses compared to vaccines containing spike with high mannose (HM) or paucimannose (PM) glycans. | Communications Medicine

Fig. 3: SLA archaeosome-adjuvanted T4 foldon-trimerized SARS-CoV-2 spike with complex type (CT) N-linked glycans induces enhanced neutralizing responses compared to vaccines containing spike with high mannose (HM) or paucimannose (PM) glycans.

From: Modifying the glycosylation profile of SARS-CoV-2 spike-based subunit vaccines alters focusing of the humoral immune response in a mouse model

Fig. 3

C57BL/6 mice (n  =  10/group) were immunized i.m. with SmT2, either CT or HM, or Sm-gp41 (PM), adjuvanted with SLA archaeosomes on days 0 and 21. a Splenocytes were harvested on Day 28 and analyzed by IFN-γ ELISpot when stimulated by spike peptide pools. Values obtained with media alone were subtracted from those measured in the presence of the peptides. b Serum was collected and analyzed by ELISA against SmT1 (CT) to determine the antibody titers on Day 20 and Day 28. c Serum was collected on Day 28 and analyzed for neutralizing activity against reference SARS-CoV-2 spike in a surrogate cell-based SARS-CoV-2 spike-hACE2 binding assay. In brief, labeled recombinant spike trimers, mouse serum (diluted 1 in 75) and hACE2-expressing cells were co-incubated and the spike-hACE2 interactions were quantified by flow cytometry. In all graphs, statistical significance of differences is shown as: *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 by one-way ANOVA followed by Tukey’s multiple comparisons test. IFNγ: Interferon gamma; Bkgd: background; CT: Complex-type; HM: High mannose; PM: Paucimannose; GMT: Geometric Mean Titer; OD: Optical Density.

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