Fig. 1: Tetravalent gp120 Env immunization.
From: Convergence and divergence of B cell responses in two HIV-1 Env immunizations in Rhesus macaques
a NHP immunization scheme with multivalent HIV-1 gp120 Env cocktails delivered as DNA and monomeric proteins. i.d. intradermal, i.m. intramuscular. b Viremia time-course of the four NHPs included in this study post-seroconversion or after the fifteenth challenge for RVv15. Each NHP is color-coded as follows: red circles: RNs14; blue squares: RPt15; orange triangles: RSf15; green inverted triangles: RSf15. Dashed line at assay limit of sensitivity threshold (60 copies/ml). Measurements were performed in technical duplicates. Also see Supplementary Data 1. c Serum was collected four weeks prior to immunization and again at weeks 10 and 18 post-immunization. Peak serum neutralization data, defined as highest ID50 value within the time course, are shown for the four NHPs in this study against two tier 1 viruses (C.MW965.26 and TH023.6) and four tier 2 viruses (A.92UG037.1, B.JR-FL, B.WITO4160.33, and C.Du172.17). Each NHP is color-coded as indicated in panel b. Lines indicate group mean and standard deviation. Measurements were performed in technical duplicates. Also see Supplementary Data 2. d Immunogen binding profiles of 734 B cells isolated from all four NHPs. Reactivity with individual and multiple gp120 Env immunogens is color coded as indicated: yellow: A.92UG037 gp120 only; orange: B.JR-FL gp120 only; red: C.93MW965 gp120 only; brown: AE.consensus gp120 only; light blue: 2 gp120 Envs; violet: 3 gp120 Envs; dark blue: all 4 gp120 Envs. Also see Supplementary Data 3. e Binding to individual immunogens of monoreactive B cells, expressed as percentage of the total number of monoreactive B cells. Data are shown as aggregate (“All NHPs”) and for each NHP. Reactivity with individual and multiple gp120 Env immunogens is color coded as indicated in panel d. Also see Supplementary Data 4. f Immunogen cross-reactivity of multireactive B cells, expressed as percentage of the total number of multireactive B cells, shown as aggregate (“All NHPs”) and for each NHP. Reactivity with individual and multiple gp120 Env immunogens is color coded as indicated in panel d. Also see Supplementary Data 5.
