Fig. 3: Somatic hypermutation frequencies of IgVH gene segments isolated from memory B cells collected after the end of the sequential immunization with IDLV expressing CH505 Envs.
From: Convergence and divergence of B cell responses in two HIV-1 Env immunizations in Rhesus macaques
We analyzed a total of 150 IgVHDJH sequences from memory B cells isolated at week 117 that bound to either all immunogens (n = 20, left column) or to the CH505.wk136 SOSIP-stabilized trimer immunogen only (n = 130, right column). IgVH gene segment somatic hypermutation frequencies (y-axis) were calculated using Cloanalyst (see methods). Lines show the mean and standard deviation for each group. Statistically significant difference between the two groups (p = 0.0001) was evaluated using the non-parametric, two-tailed Mann-Whitney test. The NHP source for each sequence is coded as follows: red triangles: Rh6575; blue squares: Rh6600; black circles: Rh6601. Also see Supplementary Data 8 and 9.
