Fig. 2: Enantiomer-specific effects of NPB on cancer cell viability and BADS99 dephosphorylation. | Communications Medicine

Fig. 2: Enantiomer-specific effects of NPB on cancer cell viability and BADS99 dephosphorylation.

From: Reply to: “The non-cytotoxic small molecule NPB does not inhibit BAD phosphorylation and forms colloidal aggregates”

Fig. 2

A NPB enantiomer. S (levorotatory) and R (dextrorotatory). B Cell viability assay: Dose-response curve of NPB. The cell survival fraction (%) was measured at 72 h (n = 3). The IC50 value was calculated using GraphPad Prism (7.0). C Western blot: The levels of pBADS99 and BAD protein in MCF-7 or A2780 cells treated with doses of NPB-racemic mix (1:1) as indicated for 72 h were evaluated using WB analysis. β-ACTIN was used as an input control. D Cell viability (%) of MCF-7 and A2780 cells was measured by AlamarBlue assay after 72 h of treatment with variable ratios of NPB enantiomers (n = 3). The unprocessed western blot images are represented in Supplementary Fig. 3.

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