Extended Data Fig. 2: Multiplex genome integration efficiency.
From: Biosynthesis of catharanthine in engineered Pichia pastoris
Multiplex genome integration efficiency. mVenus, mCherry, and HygB were chosen as the reporter genes to test the efficiency of editing several loci simultaneously. For two-loci integration, mCherry (TEF1p-mCherry-PRX5t) and mVenus (GAPp-mVenus-AOX1t) were used as reporters and the genome integration efficiency was calculated as the percentage of cells showing both mCherry and mVenus fluorescence. For three-loci integration, mCherry (TEF1p-mCherry-PRX5t), mVenus (GAPp-mVenus-AOX1t), and HygB (TEF1p-HygB-PRX5t) were used as reporters and the genome integration efficiency was calculated as the percentage of colonies showing mCherry and mVenus fluorescence as well as hygromycin resistance. The results represent the mean ± s.d. of biological triplicates (n = 3).
