Extended Data Fig. 4: R-SMC gene expresson. | Nature Cardiovascular Research

Extended Data Fig. 4: R-SMC gene expresson.

From: Smad3 regulates smooth muscle cell fate and mediates adverse remodeling and calcification of the atherosclerotic plaque

Extended Data Fig. 4

(A) Expression of Mmp3 in lineage labeled (Cre positive) and non-lineage labelled (Cre negative) cells based on scRNAseq data. (B) Measured Mmp3 activity detected in isolated aortic tissue from wild type (brown) vs Smad3ΔSMC mice (orange). (C) Featureplot of lineage-traced cells expressing Mmp3 in control and Smad3ΔSMC aortic root. (D) High magnification of a section of atherosclerotic plaque with region of broken elastic lamina stained for Mmp3 expression (orange-brown color). Scale bar: 75um. (E) Fraction of transition SMC in control and Smad3ΔSMC lesions with R-SMC fate as defined by unbiased clustering (left) and by concurrent high Mmp3 and CxCl12 expression (right). (F) Number of migrated THP-1 cells after 3 hours of transwell-incubation with no cells, control HCASMC, and SMAD3-deficient HCASMC.

Back to article page