Extended Data Fig. 8: AAV-BI30-mediated gene transfer enables long-term transgene expression in CNS endothelial cells.
From: A high-efficiency AAV for endothelial cell transduction throughout the central nervous system
AAV-BI30:CAG-NLS-GFP-miR122-WPRE was intravenously administered to adult C57BL/6 mice at 1 × 1011 vg/animal and transduction was assessed after approximately 5 months (152 days). Vascular counterstains displayed are as follows: ICAM2 in brain parenchyma and spinal cord; isolectin in retina; and ERG in pia vasculature. High-zoom colocalization with GFP shown in rightmost column. Scale bars are as follows: middle row 200 μm; rightmost row 50 μm. Images are representative of n = 3 mice.
