Extended Data Fig. 10: Comparison of engineered AAV capsids with enhanced CNS endothelial cell transduction.
From: A high-efficiency AAV for endothelial cell transduction throughout the central nervous system
The heptameric peptides used to generate the listed capsids were uniformly inserted within the surface exposed hypervariable region VIII of the AAV sequence67. Specifically, heptamers are inserted between amino acids 587 & 588 in AAV-PPS and 588 & 589 in AAVs BI30, BR1, PHP.B, PHP.V1, and PHP.V2. AAV-PHP.eB shares AAV-PHP.B’s TLAVPFK insert and additionally contains an AQ to DG mutation at amino acids 587-588 (bracketed sequence).
