Extended Data Fig. 1: AAV-BI30 is more efficient at transducing hCMEC/D3 cells than AAV9 across a wide range of doses.
From: A high-efficiency AAV for endothelial cell transduction throughout the central nervous system
hCMEC/D3 cells were grown to confluence in a 24-well plate format. AAV9 or AAV-BI30 carrying a CAG−NLS-GFP-miR122-WPRE genome was applied to cells at 0, 500, 1,000, 5,000, 10,000, or 50,000 vg/cell. 4 days post-treatment the cells were analyzed for GFP expression via flow cytometry. The profiles show data from a single sample from each condition but are representative of n = 2 replicates.
