Extended Data Fig. 7: PDGFR-β knockout reduces vascular density and inhibits Ki67, Snail and FSP-1 expression in MI- associated ECs. | Nature Cardiovascular Research

Extended Data Fig. 7: PDGFR-β knockout reduces vascular density and inhibits Ki67, Snail and FSP-1 expression in MI- associated ECs.

From: Endothelial plasticity drives aberrant vascularization and impedes cardiac repair after myocardial infarction

Extended Data Fig. 7

MI was induced in WT and PDGFR-β-ΔEC mice. Hearts were excised 3 weeks after surgery. MI tissue sections were immunostained with (a-c) anti-Ki67 and anti-CD31, (d) anti-vWF and anti-Snail, or (e) anti-CD31 and anti-FSP-1 antibodies. a, Representative immunofluorescence images in infarct zone are shown (n = 3 mice). Scale bar: 100 μm. b,c, quantified results (mean ± SEM, n = 3 mice). b, Density of CD31+ cells. AU, arbitrary unit. c, Ki67 expression in CD31+ cells. Statistical analysis by unpaired two-tailed Students’ t test. d,e, Representative immunofluorescence images in infarct zone are shown (n = 5 mice). Scale bar: 100 μm.

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