Extended Data Fig. 10: PDGFR inhibition improves cardiac function recovery and promotes tissue repair after MI.
MI was induced in mice, followed by administration with saline or 15 mg/kg crenolanib. a,b, Cardiac function was analyzed by echocardiogram (n = 11 mice). Representative images are shown. a, Long-axis echocardiogram analysis. Scale bar: 2 mm. b, M mode echocardiogram analysis. Scale bar: 2 mm. Arrows indicate myocardial anterior walls. c,d, Heart tissues were harvested 3 weeks after MI induction. Cardiac sections were stained with Masson’s trichrome stain. c, Representative images are shown (n = 4 mice). Scale bar: 500 μm. Arrows indicate epicardium. d, Quantified cardiomyocyte area (mean ± SEM, n = 4-5 mice). Statistical analysis by unpaired two-tailed Students’ t test. e, Heart tissues were harvested 3 weeks after MI induction. Cardiac sections were stained with an anti-CD31 antibody and analyzed by immunofluorescence imaging. Vascular density was quantified (mean ± SEM, n = 5 mice). Statistical analysis by two-way ANOVA.
