Extended Data Fig. 1
From: Syndecan-2 selectively regulates VEGF-induced vascular permeability
a-b, western blot analysis of VEGFR2 phosphorylation (pVEGFR2) following stimulation with VEGFA165 (50 ng/ml) or VEGFA121 (50 ng/ml) in Control (Ctr Si) vs. Sdc2-depleted (Sdc2 siRNA) HUVEC. c, VEGFA165 -induced Evans blue leakage in the back skin of WT vs Sdc2iECKO mice (n = 5–6) (representative blots of 3 independent experiments). d, evaluation of baseline permeability in various organs measured as Evans blue dye leakage in Wild type (WT) vs Sdc2-/- mice (n = 3–6). e, f western blot analysis of cell surface protein levels in Control (Ctr) vs. Sdc2-depleted (Sdc2 siRNA) HUVEC (n = 3). Data are presented as mean values + /- SEM (standard error of the mean). In all figure panels, each dot represents a biological independent experiment (n). Statistical analysis was performed by one-way ANOVA with Sidak’s multiple comparison test (panels c, d), and by unpaired t-student test (panel f), (N.S. not significant, * P < 0.05, ** P < 0.01, *** P < 0.001).
