Extended Data Fig. 3
From: Syndecan-2 selectively regulates VEGF-induced vascular permeability
a, position and sequence of DEP1-binding motif in human Sdc2. b, direct ELISA comparing Sdc2 pAb (5 µg/ml) binding to mouse Sdc2 vs. mouse Sdc4. c, bEnd.3 cell proliferation in presence of rabbit IgG (5 µg/ml) or Sdc2 pAb (5 µg/ml) measured with xCELLigence system. d-e, ECs migration measured by in vitro wound-healing assay in bEnd.3 cells in presence of rabbit IgG (5 µg/ml) or Sdc2 pAb (5 µg/ml) (n = 3–4) f-g, TTC staining (at 24 h post stroke) and quantification of stroke infarct in WT vs Sdc2iECKO (n = 5–8). h-i, TTC staining (at 24 h post stroke) in Sdc2-/- mice with or without Sdc2 pAb treatment (administered 1 hour before stroke) (n = 4–9). Data are presented as mean values + /- SEM (standard error of the mean). In all figure panels, each dot represents a biological independent experiment (n). Statistical analysis was performed by one-way ANOVA with Sidak’s multiple comparison test (panels e, i), and by unpaired t-student test (panel g), (N.S. not significant, * P < 0.05, ** P < 0.01, *** P < 0.001).
