Extended Data Fig. 2: Validation of separation between cardiomyocyte and stromal cell preparations.

(a-c) Cardiomyocytes (CMs) and stromal cells isolated from P1 and P7 hearts were analyzed by RT-PCR for markers of the major cardiac cell types (n = 12 mice), namely (a) cardiomyocytes (cTnT, cardiac troponin T), (b) endothelial cells (Pecam) and (c) fibroblasts (Ddr2), showing the absence of stromal cell markers in cardiomyocyte preparations. In all panels, numerical data are presented as mean (error bars show s.e.m.); statistical significance was determined using one-way ANOVA followed by Tukey’s test.